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anti a2b5 magnetic beads  (Miltenyi Biotec)


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    Miltenyi Biotec anti a2b5 magnetic beads
    Anti A2b5 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a2b5/Anti-A2B5+MicroBeads%2C+human%2C+mouse/pmc12869229-54-8-17
    Average 94 stars, based on 22 article reviews
    anti a2b5 magnetic beads - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Deciphering the Action of Neuraminidase in Glioblastoma Models.
    Article Snippet: .. Flow cytometry was performed using MACSQuant® 10 (Miltenyi Biotec SAS, Paris, France) on dissociated cells using A2B5 (mouse APC-IgM, clone 105, Miltenyi Biotec SAS) following the manufacturer’s instructions. .. Data were analyzed using FlowJo software (10.8.1, Becton Dickinson & Company, Tree Star, Inc., Ashland, OR, USA).

    Article Title: Mitogenic and progenitor gene programmes in single pilocytic astrocytoma cells
    Article Snippet: .. Randomization This is not relevant to the current study because no randomization or treatment selection was necessary Blinding Not relevant because a sample response based on treatment/conditions was not done Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used A2B5 clone: 105HB29, Miltenyi Biotec. .. F-IHC antibody panel consisted of Olig2 (R&D System, Goat polyclonal, AF2418) with 3 nature research | reporting sum m ary April 2018 CY5.5, GFAP (Cell Signaling Techology, Mouse monoclonal, Clone GA5, #3670) with FITC, and Ki-67 (Dako, Mouse monoclonal, Clone MIB-1, M7240) with CY3.

    Article Title: Deciphering the Action of Neuraminidase in Glioblastoma Models
    Article Snippet: .. Flow cytometry was performed using MACSQuant ® 10 (Miltenyi Biotec SAS, Paris, France) on dissociated cells using A2B5 (mouse APC-IgM, clone 105, Miltenyi Biotec SAS) following the manufacturer’s instructions. .. Data were analyzed using FlowJo software (10.8.1, Becton Dickinson & Company, Tree Star, Inc., Ashland, OR, USA).

    Article Title: Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes.
    Article Snippet: .. For flow cytometry analysis, cells at DIV1 before or after immune-magnetic bead selection were stained with LIVE/ DEAD fixable Aqua Dead Cell Stain kit (ThermoFisher Scientific, Eugene, OR), blocked for 15 minutes at þ4 C with human FcR blocking reagent, and immunostained with A2B5, O4 (Miltenyi, Somerville, MA), PDGFRa (Cell Signaling, Whitby, Ontario), and/or IL-7 receptor (IL-7R) antibodies (BioLegend, San Diego, CA). .. Data were acquired on a BD LSR II (BD Biosciences, San Jose, CA) and analysis carried out with FlowJo software.

    Marker:

    Article Title: Phenotyping clonal populations of glioma stem cell reveals a high degree of plasticity in response to changes of micro-environment
    Article Snippet: .. Antibody targets for surface marker phenotyping were CD44 (Miltenyibiotec, 130-113-332), CD133 (BD,747641), A2B5 (Miltenyibiotec, 130-093-582) and CD15 (BD, 563838). .. Data acquisition was performed on a CytoFLEX-S (Beckman-Coulter, High-Wycombe, UK) cell analyser with data analysis done on CytExpert (Beckman-Coulter, High-Wycombe, UK) and FlowjoTM.

    Selection:

    Article Title: Mitogenic and progenitor gene programmes in single pilocytic astrocytoma cells
    Article Snippet: .. Randomization This is not relevant to the current study because no randomization or treatment selection was necessary Blinding Not relevant because a sample response based on treatment/conditions was not done Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used A2B5 clone: 105HB29, Miltenyi Biotec. .. F-IHC antibody panel consisted of Olig2 (R&D System, Goat polyclonal, AF2418) with 3 nature research | reporting sum m ary April 2018 CY5.5, GFAP (Cell Signaling Techology, Mouse monoclonal, Clone GA5, #3670) with FITC, and Ki-67 (Dako, Mouse monoclonal, Clone MIB-1, M7240) with CY3.

    Article Title: SARM1 detection in myelinating glia: sarm1 / Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice.
    Article Snippet: Subsequently, red blood cell lysis buffer (BD Biosciences, #555899) was used to remove red blood cells. .. OPCs were isolated by positive selection using 2.5 μg A2B5 (Merck Millipore, #MAB312) primary antibody, followed by 20 μL of rat anti-mouse IgM antibody (Miltenyi, #130-047-302) per brain using the MACS protocol according to the manufacturer’s instructions. .. To collect the A2B5+ fraction, the MACS MS column (Miltenyi, #130-042-201) was removed from the magnetic stand (Miltenyi, #130-042-102) and cells were flushed from the column with 1 mL of prewarmed OPC medium (DMEM F/12 containing N-Acetyl cysteine (60 μg mL−1, Sigma, #A9165), human recombinant insulin (10 μg mL−1), sodium pyruvate (1 mM, Thermo Fisher, #11360-070), apotransferrin (50 μg mL−1, Sigma, #T2036), putrescine (16.1 μg mL−1, Sigma, #P7505), sodium selenite (40 ng mL−1, Sigma, #S5261), progesterone (60 ng mL−1, Sigma, #P0130), bovine serum albumin (330 μg mL−1, Sigma, #A4919).

    Article Title: Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes.
    Article Snippet: .. For flow cytometry analysis, cells at DIV1 before or after immune-magnetic bead selection were stained with LIVE/ DEAD fixable Aqua Dead Cell Stain kit (ThermoFisher Scientific, Eugene, OR), blocked for 15 minutes at þ4 C with human FcR blocking reagent, and immunostained with A2B5, O4 (Miltenyi, Somerville, MA), PDGFRa (Cell Signaling, Whitby, Ontario), and/or IL-7 receptor (IL-7R) antibodies (BioLegend, San Diego, CA). .. Data were acquired on a BD LSR II (BD Biosciences, San Jose, CA) and analysis carried out with FlowJo software.

    Chromatin Immunoprecipitation:

    Article Title: Mitogenic and progenitor gene programmes in single pilocytic astrocytoma cells
    Article Snippet: .. Randomization This is not relevant to the current study because no randomization or treatment selection was necessary Blinding Not relevant because a sample response based on treatment/conditions was not done Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used A2B5 clone: 105HB29, Miltenyi Biotec. .. F-IHC antibody panel consisted of Olig2 (R&D System, Goat polyclonal, AF2418) with 3 nature research | reporting sum m ary April 2018 CY5.5, GFAP (Cell Signaling Techology, Mouse monoclonal, Clone GA5, #3670) with FITC, and Ki-67 (Dako, Mouse monoclonal, Clone MIB-1, M7240) with CY3.

    Magnetic Resonance Imaging:

    Article Title: Mitogenic and progenitor gene programmes in single pilocytic astrocytoma cells
    Article Snippet: .. Randomization This is not relevant to the current study because no randomization or treatment selection was necessary Blinding Not relevant because a sample response based on treatment/conditions was not done Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used A2B5 clone: 105HB29, Miltenyi Biotec. .. F-IHC antibody panel consisted of Olig2 (R&D System, Goat polyclonal, AF2418) with 3 nature research | reporting sum m ary April 2018 CY5.5, GFAP (Cell Signaling Techology, Mouse monoclonal, Clone GA5, #3670) with FITC, and Ki-67 (Dako, Mouse monoclonal, Clone MIB-1, M7240) with CY3.

    Isolation:

    Article Title: SARM1 detection in myelinating glia: sarm1 / Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice.
    Article Snippet: Subsequently, red blood cell lysis buffer (BD Biosciences, #555899) was used to remove red blood cells. .. OPCs were isolated by positive selection using 2.5 μg A2B5 (Merck Millipore, #MAB312) primary antibody, followed by 20 μL of rat anti-mouse IgM antibody (Miltenyi, #130-047-302) per brain using the MACS protocol according to the manufacturer’s instructions. .. To collect the A2B5+ fraction, the MACS MS column (Miltenyi, #130-042-201) was removed from the magnetic stand (Miltenyi, #130-042-102) and cells were flushed from the column with 1 mL of prewarmed OPC medium (DMEM F/12 containing N-Acetyl cysteine (60 μg mL−1, Sigma, #A9165), human recombinant insulin (10 μg mL−1), sodium pyruvate (1 mM, Thermo Fisher, #11360-070), apotransferrin (50 μg mL−1, Sigma, #T2036), putrescine (16.1 μg mL−1, Sigma, #P7505), sodium selenite (40 ng mL−1, Sigma, #S5261), progesterone (60 ng mL−1, Sigma, #P0130), bovine serum albumin (330 μg mL−1, Sigma, #A4919).

    Magnetic Cell Separation:

    Article Title: SARM1 detection in myelinating glia: sarm1 / Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice.
    Article Snippet: Subsequently, red blood cell lysis buffer (BD Biosciences, #555899) was used to remove red blood cells. .. OPCs were isolated by positive selection using 2.5 μg A2B5 (Merck Millipore, #MAB312) primary antibody, followed by 20 μL of rat anti-mouse IgM antibody (Miltenyi, #130-047-302) per brain using the MACS protocol according to the manufacturer’s instructions. .. To collect the A2B5+ fraction, the MACS MS column (Miltenyi, #130-042-201) was removed from the magnetic stand (Miltenyi, #130-042-102) and cells were flushed from the column with 1 mL of prewarmed OPC medium (DMEM F/12 containing N-Acetyl cysteine (60 μg mL−1, Sigma, #A9165), human recombinant insulin (10 μg mL−1), sodium pyruvate (1 mM, Thermo Fisher, #11360-070), apotransferrin (50 μg mL−1, Sigma, #T2036), putrescine (16.1 μg mL−1, Sigma, #P7505), sodium selenite (40 ng mL−1, Sigma, #S5261), progesterone (60 ng mL−1, Sigma, #P0130), bovine serum albumin (330 μg mL−1, Sigma, #A4919).

    Incubation:

    Article Title: Reprogramming of cells to a new fate
    Article Snippet: Undissociated cells were removed by passing suspensions twice through a strainer (BD). .. Cells were incubated individually with PE-conjugated SSEA1 (BD) and APC-conjugated PSA-NCAM, Prominin-1 and A2B5 (Miltenyi Biotec) antibodies for 30 min at 4° C. (all concentrations as suggested by the manufacturer). ..

    Staining:

    Article Title: Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes.
    Article Snippet: .. For flow cytometry analysis, cells at DIV1 before or after immune-magnetic bead selection were stained with LIVE/ DEAD fixable Aqua Dead Cell Stain kit (ThermoFisher Scientific, Eugene, OR), blocked for 15 minutes at þ4 C with human FcR blocking reagent, and immunostained with A2B5, O4 (Miltenyi, Somerville, MA), PDGFRa (Cell Signaling, Whitby, Ontario), and/or IL-7 receptor (IL-7R) antibodies (BioLegend, San Diego, CA). .. Data were acquired on a BD LSR II (BD Biosciences, San Jose, CA) and analysis carried out with FlowJo software.

    Blocking Assay:

    Article Title: Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes.
    Article Snippet: .. For flow cytometry analysis, cells at DIV1 before or after immune-magnetic bead selection were stained with LIVE/ DEAD fixable Aqua Dead Cell Stain kit (ThermoFisher Scientific, Eugene, OR), blocked for 15 minutes at þ4 C with human FcR blocking reagent, and immunostained with A2B5, O4 (Miltenyi, Somerville, MA), PDGFRa (Cell Signaling, Whitby, Ontario), and/or IL-7 receptor (IL-7R) antibodies (BioLegend, San Diego, CA). .. Data were acquired on a BD LSR II (BD Biosciences, San Jose, CA) and analysis carried out with FlowJo software.



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